Publications of Arne Skerra

Schmidt, T. G.M.; Koepke, J.; Frank, R.; Skerra, A.: Molecular interaction between the Strep-tag affinity peptide and its cognate target streptavidin. Journal of Molecular Biology (London) 255 (5), pp. 753 - 766 (1996)

Schiweck, W.; Skerra, A.: Fermenter Production of an Artificial Fab Fragment, Rationally Designed for the Antigen Cystatin, and Its Optimized Crystallization Through Constant Domain Shuffling. Proteins: Structure, Function, and Bioinformatics 23 (4), pp. 561 - 565 (1995)

Kleymann, G.; Ostermeier, C.; Ludwig, B.; Skerra, A.; Michel, H.: Engineered Fv Fragments as a Tool for the One-Step Purification of Integral Multisubunit Membrane Protein Complexes. Nature Biotechnology 155, pp. 155 - 160 (1995)

Dübel, S.; Breitling, F.; Kontermann, R.; Schmidt, T.G.; Skerra, A.; Little, M.: Bifunctional and multimeric complexes of streptavidin fused to single chain antibodies (scFv). Journal of Immunological Methods 178 (2), pp. 201 - 209 (1995)

Skerra, A.: Use of the tetracycline promoter for the tightly regulated production of a murine antibody fragment in Escherichia coli. Gene 151 (1-2), pp. 131 - 135 (1994)

Müller, H. N.; Skerra, A.: Grafting of a high-affinity Zn(II)-binding site on the beta-barrel of retinol-binding protein results in enhanced folding stability and enables simplified purification. Biochemistry 33 (47), pp. 14126 - 14135 (1994)

Schmidt, T. G.M.; Skerra, A.: One-step affinity purification of bacterially produced proteins by means of the "Strep tag" and immobilized recombinant core streptavidin. Journal of Chromatography A 676 (2), pp. 337 - 345 (1994)

Essen, L.-O.; Skerra, A.: The de Novo Design of an Antibody Combining Site: Crystallographic Analysis of the V_L Domain Confirms the Structural Model. Journal of Molecular Biology (London) 238 (1), pp. 226 - 244 (1994)

Skerra, A.: A general vector, pASK84, for cloning, bacterial production, and single-step purification of antibody F_ab fragments. Gene 141 (1), pp. 79 - 84 (1994)

Schmidt, F. S.; Skerra, A.: The bilin-binding protein of Pieris brassicae. cDNA sequence and regulation of expression reveal distinct features of this insect pigment protein. European Journal of Biochemistry 219 (3), pp. 855 - 863 (1994)

Essen, L.-O.; Skerra, A.: Single-step purification of a bacterially expressed antibody F_v fragment by immobilized metal affinity chromatography in the presence of betaine. Journal of Chromatography A 657 (1), pp. 55 - 61 (1993)

Müller, H. N.; Skerra, A.: Functional expression of the uncomplexed serum retinol-binding protein in Escherichia coli. Journal of Molecular Biology (London) 230 (3), pp. 725-32 - 732 (1993)

Skerra, A.: Bacterial expression of immunoglobulin fragments. Current Opinion in Immunology 5 (2), pp. 256 - 262 (1993)

Schmidt, T. G.M.; Skerra, A.: The random peptide library-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment. Protein Engineering Design and Selection 6 (1), pp. 109 - 122 (1993)

Skerra, A.: Phosphorothioate primers improve the amplification of DNA sequences by DNA polymerases with proofreading activity. Nucleic Acids Research 20 (14), pp. 3551 - 3554 (1992)

Skerra, A.: Molekulares Design von Proteinen mit neuen Bindungseigenschaften. Habilitation, Fachbereich Biochemie, Pharmazie und Lebensmittelchemie, Johann Wolfgang Goethe-Universität, Frankfurt am Main (1995)