Martin Beck – Molecular Sociology
How do molecular modules act in concert to generate complex cellular functions?
Translation dynamics in human cells visualized at high resolution reveal cancer drug action
Xing, H., Taniguchi, R., Khusainov, I., Kreysing, J.P., et al. Science (2023)
Co-translational binding of importins to nascent proteins
Seidel, M., Romanov, N., Obarska-Kosinska, A., et al. Nature (2023)
AI-based structure prediction empowers integrative structural analysis of human nuclear pores
Mosalaganti, S., Obarska-Kosinska, A., et al. Science (2022)
Nuclear pores dilate and constrict in cellulo
Zimmerli, C. E., Allegretti, M., et al. (Science 2021)
Cone-shaped HIV-1 capsids are transported through intact nuclear pores
Zila, Margiotta, et al. Cell (2021)
In situ structural analysis of SARS-CoV-2 spike reveals flexibility mediated by three hinges
Turoňová, Sikora, Schürrmann, et al. Science (2020)
In cell architecture of the nuclear pore complex and snapshots of its turnover
Allegretti, Zimmerli, et al. Nature (2020)
Benchmarking tomographic acquisition schemes for high-resolution structural biology
Turoňová et al., Nature Communications (2020)
Nuclear Pores Assemble from Nucleoporin Condensates During Oogenesis
Bernhard Hampoelz, Andre Schwarz et al. Cell (2019)
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In situ structural analysis of the human nuclear pore complex
von Appen et al. Nature (2015)
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Disentangling Genetic and Environmental Effects on the Proteotypes of Individuals
Romanov et al. Cell (2019)
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Pervasive Protein Thermal Stability Variation during the Cell Cycle
Becher, Andres-Pons, Romanov, Stein, et al. Cell (2018)
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Molecular architecture of the inner ring scaffold of the human nuclear pore complex
Kosinski, Mosalaganti, von Appen, et al. Science (2016)
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In situ architecture of the algal nuclear pore complex
Mosalaganti, Kosinski, Albert, Schaffer, et al. Nature Communications (2018)
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Pre-Assembled Nuclear Pores Insert into the Nuclear Envelope during Early Development
Hampoelz et al. Cell (2016)
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Integrated structural analysis of the human nuclear pore complex scaffold
Bui, von Appen, et al. Cell (2013)
Research Interests
Functional cellular modules have often been characterised in vitro. However, relatively little is known about their interplay and spatio-temporal arrangement in the context of living cells, i.e. their 'molecular sociology'. We use integrative, in situ structural biology techniques to study the structure, function and assembly of very large macromolecular complexes in their native environment. We rely on a diverse methodological repertoire, including cryo-EM, structural proteomics, biochemistry, imaging, animal model systems and computational modeling (Robinson et al, Nature 2007; Beck and Baumeister, TiCB 2016).
Projects
1.
Mosalaganti, S.; Obarska-Kosinska, A.; Siggel, M.; Taniguchi, R.; Turoňová, B.; Zimmerli, C. E.; Buczak, K.; Schmidt, F. H.; Margiotta, E.; Mackmull, M.-T. et al.
AI-based structure prediction empowers integrative structural analysis of human nuclear pores.
Science. 2022 June; 376 (6598), doi: 10.1126/science.abm9506.
2.
Zimmerli C. E., Allegretti M., Rantos V., Goetz S. K., Obarska-Kosinska A., Zagoriy I., Halavatyi A., Hummer G., Mahamid J., Kosinski J., Beck M.
Nuclear pores dilate and constrict in cellulo.
Science. 2021 Nov; 374 (6573). doi: 10.1126/science.abd9776.
3.
Zila, V., Margiotta, E., Turoňová, B., Müller, T.G., Zimmerli, C.E., Mattei, S., Allegretti, M., Börner, K., Rada, J., Müller, B., Lusic, M., Kräusslich, H.G., Beck, M.
Cone-shaped HIV-1 capsids are transported through intact nuclear pores.
Cell. 2021 Feb; 184, 1-15. doi:10.1016/j.cell.2021.01.025.
4.
Allegretti M., Zimmerli C. E., Rantos V., Wilfling F., Ronchi P., Fung H.K.H., Lee CW., Hagen W., Turonova B., Karius K., Börmel M., Zhang X., Müller C.W., Schwab Y., Mahamid J., Pfander B., Kosinski J., Beck M.
In-cell architecture of the nuclear pore complex and snapshots of its turnover.
Nature. 2020 Sept; 586, 796-800. doi: 10.1038/s41586-020-2670-5.
5.
Turoňová B, Sikora M, Schürmann C, Hagen WJH, Welsch S, Blanc FEC, von Bülow S, Gecht M, Bagola K, Hörner C, v Zandbergen G, Landry J, Doimo de Azevedo NT, Mosalaganti S, Schwarz A, Covino R, Mühlebach MD, Hummer G, Krijnse Locker J, Beck M.
In situ structural analysis of SARS-CoV-2 spike reveals flexibility mediated by three hinges.
Science. 2020 Oct; 370, 203-208. doi: 10.1126/science.abd5223.
6.
Nuclear Pores Assemble from Nucleoporin Condensates During Oogenesis.
Cell. 2019 Oct; 179(3):671-686.e17. doi: 10.1016/j.cell.2019.09.022.
7.
Romanov N., Kuhn M., Aebersold R., Ori A., Beck M., Bork P.
Disentangling Genetic and Environmental Effects on the Proteotypes of Individuals.
Cell. 2019 May; 177(5):1308-1318.e10. doi: 10.1016/j.cell.2019.03.015.
8.
Becher I., Andres-Pons A., Romanov N., Stein F., Schramm M., Baudin F., Helm D., Kurzawa N., Mateus A., Mackmull M.T., Typas A., Mueller C.W., Bork P., Beck M., Savitski M.M.
Pervasive Protein Thermal Stability Variation during the Cell Cycle.
Cell. 2018 May; 173(6):1495-1507.e18. doi: 10.1016/j.cell.2018.03.053.
9.
Mosalaganti S., Kosinski J., Albert S., Schaffer M., Strenkert D., Salome P.A., Merchant S.S., Plitzko J.M., Baumeister W., Engel B.D., Beck M.
In situ architecture of the algal nuclear pore complex.
Nat Commun. 2018 Jun; 9(1):2361. doi: 10.1038/s41467-018-04739-y.
10.
Mackmull M.T., Klaus B., Heinze I., Chokkalingam M., Beyer A., Russell R.B., Ori A., Beck M.
Landscape of nuclear transport receptor cargo specificity.
Mol Syst Biol. 2017 Dec; 13(12):962. doi: 10.15252/msb.20177608.
11.
Hampoelz B., Mackmull M.T., Machado P., Ronchi P., Bui K.H., Schieber N., Santarella-Mellwig R., Necakov A., Andres-Pons A., Philippe J.M., Lecuit T., Schwab Y., Beck M.
Pre-assembled Nuclear Pores Insert into the Nuclear Envelope during Early Development.
Cell. 2016 Jul; 166(3):664-678. doi: 10.1016/j.cell.2016.06.015.
12.
Ori A., Iskar M., Buczak K., Kastritis P., Parca L., Andres-Pons A., Singer S., Bork P., Beck M.
Spatiotemporal variation of mammalian protein complex stoichiometries.
Genome Biol. 2016 Mar 14;17:47. doi: 10.1186/s13059-016-0912-5.
13.
Kosinski J., Mosalaganti S., von Appen A., Teimer R., DiGuilio A.L., Wan W., Bui K.H., Hagen W.J,, Briggs J.A., Glavy J.S., Hurt E., Beck M.
Molecular architecture of the inner ring scaffold of the human nuclear pore complex.
Science. 2016 Apr 15;352(6283):363-5. doi: 10.1126/science.aaf0643.
14.
von Appen A., Kosinski J., Sparks L., Ori A., DiGuilio A.L., Vollmer B., Mackmull M.T., Banterle N., Parca L., Kastritis P., Buczak K., Mosalaganti S., Hagen W., Andres-Pons A., Lemke E.A., Bork P., Antonin W., Glavy J.S., Bui K.H., Beck M.
In situ structural analysis of the human nuclear pore complex.
Nature. 2015 Oct 1;526(7571):140-143. doi: 10.1038/nature15381.
15.
Ori A., Toyama B.H., Harris M.S., Bock T., Iskar M., Bork P., Ingolia N.T., Hetzer M.W., Beck M.
Integrated Transcriptome and Proteome Analyses Reveal Organ-Specific Proteome Deterioritation in Rats.
Cell Syst 2015, 1(3), 224–237. doi: 10.1016/j.cels.2015.08.012
16.
Bui K.H., von Appen A., DiGuilio A.L., Ori A., Sparks L., Mackmull M.T., Bock T., Hagen W., Andres-Pons A., Glavy J.S., Beck M.
Integrated structural analysis of the human nuclear pore complex scaffold.
Cell. 2013 Dec 5;155(6):1233-43. doi: 10.1016/j.cell.2013.10.055.
17.
Ori A., Banterle N., Iskar M., Andres-Pons A., Escher C., Khanh Bui H., Sparks L., Solis-Mezarino V., Rinner O., Bork P., Lemke E.A., Beck M.
Cell type-specific nuclear pores: a case in point for context-dependent stoichiometry of molecular machines.
Mol Syst Biol. 2013;9:648. doi: 10.1038/msb.2013.4.
