Publications of Christoph Reinhart

Kalavacherla, T.; Buschmann, S.; Schleker, E. S. M.; Michel, H.; Reinhart, C.: Purification and characterization of eukaryotic ATP-dependent transporters homologously expressed in Pichia pastoris for structural studies by cryo-electron microscopy. Protein Expression and Purification 204, 106230 (2023)

Schleker, E. S. M.; Buschmann, S.; Xie, H.; Welsch, S.; Michel, H.; Reinhart, C.: Structural and functional investigation of ABC transporter STE6-2p from Pichia pastoris reveals unexpected interaction with sterol molecules. Proceedings of the National Academy of Sciences of the United States of America 126 (43), pp. 8646 - 8654 (2022)

Singh, S.; Akhter, M. S.; Dodt, J.; Volkers, P.; Reuter, A.; Reinhart, C.; Krettler, C.; Oldenburg, J.; Biswas, A.: Identification of Potential Novel Interacting Partners for Coagulation Factor XIII B (FXIII-B) Subunit, a Protein Associated with a Rare Bleeding Disorder. International Journal of Molecular Sciences 20 (11), 2682 (2019)

Joedicke, L.; Mao, J.; Kuenze, G.; Reinhart, C.; Kalavacherla, T.; Jonker, H. R.A.; Richter, C.; Schwalbe, H.; Meiler, J.; Preu, J. et al.; Michel, H.; Glaubitz, C.: The molecular basis of subtype selectivity of human kinin G-protein-coupled receptors. Nature Chemical Biology 13 (3), pp. 284 - 290 (2018)

Gupta, S.; Biswas, A.; Akhter, M. S.; Krettler, C.; Reinhart, C.; Dodt, J.; Reuter, A.; Philippou, H.; Ivaskevicius, V.; Oldenburg, J.: Revisiting the mechanism of coagulation factor XIII activation and regulation from a structure/functional perspective. Scientific Reports 6, pp. 1 - 16 (2016)

Bevans, C. G.; Krettler, C.; Reinhart, C.; Watzka, M.; Oldenburg, J.: Phylogeny of the Vitamin K 2,3-Epoxide Reductase (VKOR) Family and Evolutionary Relationship to the Disulfide Bond Formation Protein B (DsbB) Family. nutrients 7 (7), pp. 6224 - 6249 (2015)

Krettler, C.; Bevans, C. G.; Reinhart, C.; Watzka, M.; Oldenburg, J.: Tris(3-hydroxypropyl)phosphine is superior to dithiothreitol for in vitro assessment of vitamin K 2,3-epoxide reductase activity. Analytical Biochemistry 474, pp. 89 - 94 (2015)

Bevans, C. G.; Krettler, C.; Reinhart, C.; Tran, H.; Koßmann, K.; Watzka, M.; Oldenburg, J.: Determination of the warfarin inhibition constant K_i for vitamin K 2,3-epoxide reductase complex subunit-1 (VKORC1) using an in vitro DTT-driven assay. Biochimica et Biophysica Acta-General Subjects 1830 (8), pp. 4202 - 4210 (2013)

Xia, H.; Liu, L.; Reinhart, C.; Michel, H.: Heterologous expression of human Neuromedin U receptor 1 and its subsequent solubilization and purification. Biochimica et Biophysica Acta-Biomembranes 1778 (10), pp. 2203 - 2209 (2008)

Lopez, J. J.; Shukla, A. K.; Reinhart, C.; Schwalbe, H.; Michel, H.; Glaubitz, C.: The Structure of the Neuropeptide Bradykinin Bound to the Human G-Protein Coupled Receptor Bradykinin B2 as Determined by Solid-State NMR Spectroscopy. Angewandte Chemie 120 (9), pp. 1692 - 1695 (2008)

Chillakuri, C. R.; Reinhart, C.; Michel, H.: C-terminal truncated cannabinoid receptor 1 co-expressed with G protein trimer in Sf9 cells exist in a precoupled state and show constitutive activity. The FEBS Journal 274 (23), pp. 6106 - 6115 (2007)

Shukla, A. K.; Haase, W.; Reinhart, C.; Michel, H.: Heterologous expression and characterization of the recombinant bradykinin B₂ receptor using the methylotrophic yeast Pichia pastoris. Protein Expression and Purification 55 (1), pp. 1 - 8 (2007)

Shukla, A. K.; Haase, W.; Reinhart, C.; Michel, H.: Heterologous expression and comparative characterization of the human neuromedin U subtype II receptor using the methylotrophic yeast Pichia pastoris and mammalian cells. The International Journal of Biochemistry & Cell Biology 39 (5), pp. 931 - 942 (2007)

Zeder-Lutz, G.; Cherouati, N.; Reinhart, C.; Pattus, F.; Wagner, R.: Dot-blot immunodetection as a versatile and high-throughput assay to evaluate recombinant GPCRs produced in the yeast Pichia pastoris. Protein Expression and Purification 50 (1), pp. 118 - 127 (2006)

Shukla, A. K.; Haase, W.; Reinhart, C.; Michel, H.: Functional Overexpression and Characterization of Human Bradykinin Subtype 2 Receptor in Insect Cells Using the Baculovirus System. Journal of Cellular Biochemistry 99 (3), pp. 868 - 877 (2006)

Shukla, A. K.; Reinhart, C.; Michel, H.: Comparative analysis of the human angiotensin II type 1a receptor heterologously produced in insect cells and mammalian cells. Biochemical and Biophysical Research Communications 349 (1), pp. 6 - 14 (2006)

Shukla, A. K.; Reinhart, C.; Michel, H.: Dimethylsulphoxide as a tool to increase functional expression of heterologously produced GPCRs in mammalian cells. FEBS Letters 580 (17), pp. 4261 - 4265 (2006)

Lundstrom, K.; Wagner, R.; Reinhart, C.; Desmyter, A.; Cherouati, N.; Magnin, T.; Zeder-Lutz, G.; Courtot, M.; Prual, C.; André, N. et al.; Hassaine, G.; Michel, H.; Cambillau, C.; Pattus, F.: Structural genomics on membrane proteins: comparison of more than 100 GPCRs in 3 expression systems. Journal of Structural and Functional Genomics 7 (2), pp. 77 - 91 (2006)

André, N.; Cherouati, N.; Prual, C.; Steffan, T.; Zeder-Lutz, G.; Magnin, T.; Pattus, F.; Michel, H.; Wagner, R.; Reinhart, C.: Enhancing functional production of G protein-coupled receptors in Pichia pastoris to levels required for structural studies via a single expression screen. Protein Science 15 (5), pp. 1115 - 1126 (2006)

Shukla, A. K.; Haase, W.; Reinhart, C.; Michel, H.: Biochemical and pharmacological characterization of the human bradykinin subtype 2 receptor produced in mammalian cells using the Semliki Forest virus system. Biological Chemistry 387 (5), pp. 569 - 576 (2006)

Hassaine, G.; Wagner, R.; Kempf, J.; Cherouati, N.; Hassaine, N.; Prual, C.; André, N.; Reinhart, C.; Pattus, F.; Lundstrom, K.: Semliki Forest Virus vectors for overexpression of 101 G Protein-coupled receptors in mammalian host cells. Protein Expression and Purification 45 (2), pp. 343 - 351 (2006)

Krettler, C.; Reinhart, C.; Bevans, C. G.: Expression of GPCRs in Pichia pastoris for Structural Studies. In: Methods in Enzymology, Vol. 520, pp. 1 - 29 (Ed. Conn, P. M.). Academic Press, Elsevier, Burlington (2013)

Reinhart, C.; Krettler, C.: Das Pichia pastoris Expressionssystem. In: Gentechnische Methoden, pp. 431 - 453 (Eds. Jahnson, M.; Rothhämel, S.). Spektrum Akademischer Verlag, Heidelberg (2011)

Dierl, S.; Reinhart, C.; Reiländer, H.: Das Pichia pastoris Expressionssystem. In: Gentechnische Methoden, Eine Sammlung von Arbeitsanleitungen für das molekularbiologische Labor, pp. 335 - 352 (Ed. Jansohn, M.). Spektrum Akademischer Verlag, München (2006)

Reinhart, C.; Krettler, C.: Expression of membrane proteins in yeasts. In: Structural Genomics on Membrane Proteins, pp. 115 - 152 (Ed. Lundstrom, K. H.). Taylor & Francis, Boca Raton, FL, USA (2006)

Reinhart, C.; Weiss, H. M.; Reiländer, H.: Purification of an Affinity-Epitope Tagged G-Protein Coupled Receptor. In: Membrane Protein Purification and Crystallization., 9, pp. 167 - 178 (Eds. Hunte, C.; Schägger, H.; von Jagow, G.). Academic Press/Elsevier Science, San Diego, CA, U.S.A. (2003)

Reinhart, C.: Charakterisierung, Solubilisierung und Reinigung eines ß_2-adrenergen Rezeptors nach heterologer Produktion in der methylotrophen Hefe Pichia pastoris. Dissertation, 149 pp., Fachbereich Chemische und Pharmazeutische Wissenschaften, Johann Wolfgang Goethe-Universität, Frankfurt am Main (2001)

Reinhart, C.: Gezielte Mutagenese des calciumbindenden Proteins Calsequestrin aus dem Kaninchen-Skelettmuskel. Diploma, 116 pp., Fachbereich Biologie, Johann Wolfgang Goethe-Universität, Frankfurt am Main (1997)

Lopez, J. J.; Shukla, A. K.; Reinhart, C.; Schwalbe, H.; Michel, H.; Glaubitz, C.: Innentitelbild: The Structure of the Neuropeptide Bradykinin Bound to the Human G‐Protein Coupled Receptor Bradykinin B2 as Determined by Solid‐State NMR Spectroscopy, Angewandte Chemie 120 (9 Ed.), p. 1547 - 1547 (2008)